The cells were analysed with a Flow Cytometer GalliosTM Beckman Coulter. Flow cytometry protocols were developed to detect oxidative stress. The p53p21WAFCIP Pathway Mediates Oxidative Stress and. Core Facilities Dana-Farber Cancer Institute Boston MA. Cellular citrate synthase activity was measured using the protocol previously described 21. Accumulation of Non-Superoxide Anion Reactive Oxygen.